freshers And Experience

Greetings from Hemmo Pharmaceuticals Pvt Ltd.

we are USFDA Approved API MAnufacturer.

WE have opening for Quality Control Department

1) QC Chemist / Officer (HPLC Analysis ) 3-5 years of work Experience.

2) Microbiologist, Freshers/ 1 year of Experience.

Also Exp in USFDA Approved API Pharma must.

note: shift duty

job location: turbhe

send CV : hr@hemmopharma.com

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Yeast two hybrid assay ?

Yeast two hybrid assay: - Study interaction, detecting protein-protein interaction. Principle of the two-hybrid: - A] Standard model of transcription activator: - - The DNA binding domain of an activator binds to an enhancer. - The activating domain interacted with the based complex. Recruiting it the promoter B] Two hybrid assay for protein-protein interaction using gene clone technique linked the gene one protein to the part of a gene encoding a DNA binding domain, to encode 1 hybrid protein, linked the gene for another protein to the part of the gene encoding a transcription activating domain to encode a two hybrid proteins are introduce into yeast...

RFLP (Restriction Fragment Length Polymerization)

RFLP (Restriction Fragment Length Polymerization) Principle: - Restriction fragment length polymerization (RFLP) technique in which organism may be differentiated by analysis of pattern derived from cleavages of this DNA - If two organism differ in this distance between sites of cleavage of particular restriction endonucleases, the length of the fragment produced wise differ when the DNA is digested with a restriction enzyme. - The similarity of the pattern generated can be used to differentiated species (and even strain) from one other. Introduction: - Alc Jeffery in 1985 discovered the technique called restriction fragment length polymerization (RFLP) - RFLP is t...

What is random priming?|What is non radioactive Labelling?|What are random primers used for?

Random primer labelling (primer extension) Ø Gene probes, clone or TCR amplified and oligonucleotide probes can be random prime labelled with radioactive isotopes and non-radioactive labelled DIG Ø Random prime labelling of DNA fragment (double or single stranded DNA was developed by Fein Berg and Bolgesteinds an alternative to nicker translation to produce uniformly labelled probes. Ø Double stranded DNA is degenerated and aneled with random oligonucleotide primer. Ø The oligonucleotide primer too 5`―› 3` polymerase which synthesis labelled probe in presence of labelled nucleotide pressure. PCR DIG labelling: - DIG DUTP is incorporated during PCR cycle into the DNA strands amplified from the DNA target. Photo biotin labelling: - Photo biotin labelling is a chemical reaction not an enzymatic one. ...

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