freshers And Experience

Greetings from Hemmo Pharmaceuticals Pvt Ltd.

we are USFDA Approved API MAnufacturer.

WE have opening for Quality Control Department

1) QC Chemist / Officer (HPLC Analysis ) 3-5 years of work Experience.

2) Microbiologist, Freshers/ 1 year of Experience.

Also Exp in USFDA Approved API Pharma must.

note: shift duty

job location: turbhe

send CV : hr@hemmopharma.com

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Yeast two hybrid assay ?

Yeast two hybrid assay: - Study interaction, detecting protein-protein interaction. Principle of the two-hybrid: - A] Standard model of transcription activator: - - The DNA binding domain of an activator binds to an enhancer. - The activating domain interacted with the based complex. Recruiting it the promoter B] Two hybrid assay for protein-protein interaction using gene clone technique linked the gene one protein to the part of a gene encoding a DNA binding domain, to encode 1 hybrid protein, linked the gene for another protein to the part of the gene encoding a transcription activating domain to encode a two hybrid proteins are introduce into yeast...

Chromatin Immunoprecipitation (CHIP)|What is a ChIP assay?|How does Chipseq work?

Chromatin Immunoprecipitation (CHIP) Ø These are numerous protein that function by interacting directly with DNA, such chromatin protein or the factor that perform replication, repair and transcription Ø It is critical to map the dynamics of protein-DNA interaction in living cell in order to fully understand the complex function. Ø The powerful technique of chromatin immunoprecipitation (CHIP) was developed to capture such interaction. Ø CHIP allows to reaches to detect the presence of any protein of interest at a specific DNA sequence in vivo. Ø It allows the detection of specific protein-DNA interaction in vivo. Ø CHIP on CHIP or CHIP sequence allows mapping of all the protein-binding sites for a given protein across the entire genome. Ø This method depends on the use of an antibody to detect the protein intere...

DMS footprinting?|DMS footprinting method?|how DMS footprinting works?

DMS footprinting - Dimethyl sulphate (DMS) is one of the oldest and most versatile chemical reagents used to probe RNA structure. - It was introduced for RNA structure mapping in 1980. This early method allowed detection of methylation by DMS at N 7 of guanosine and N 3 of cytidine nucleotide because of modification facilitate cleavage of the chain. - The sites of the methylation were determined by polyacrylamide gel electrophoresis (PAGE) analysis of end-labelled RNA. - Analogous method was used to footprint the binding of protein to DNA. - ...

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