Epitope Tagging 1. Epitope tagging is used for localization and purification of protein. 2. Using standard engineering techniques, a short peptide tag can be added to protein interest. 3. It is used to study protein-protein or DNA-protein interaction. 4. If a tag itself is an antigen determinant or epitope it can be targeted by an appropriate commercially available antibody. 5. The antibody, suitably labelled can be used to determine the location of protein in cell to purify it by immunoprecipitation of affinity chromatography. 6. In an immunoprecipitation antibody directed against the epitope tag re added to a solution as antibody protein complex. 7. Richard young and his co-workers originally identified 10 polypeptide that are authentic polymerase 2 sub at least tightly bond contained. 8. The method they used is called epitope tagging, in which they attach a small foreign ep