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Polymerase Chain Reaction
Polymerase chain reaction is method
widely used in molecular biology.
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To make several copies to specific
DNA fragment using PCR copies.
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Copies of DNA is exponentially
amplified to generate thousand to millions of more copies of that particular
DNA segment.
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PCR is common technique and this
technique is used in medical laboratory and clinical laboratory.
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PCR was developed by Kary mullis in
1983.
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He was awarded the Nobel prize in
chemistry in 1993.
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Hybridization probes for southern or northern
hybridization and DNA cloning. Which requires large amount of DNA.
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Representing specific DNA region.
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PCR supplies these techniques with
high amounts of pure DNA, enabling analysis of DNA samples even from very small
amount of starting material.
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PCR includes DNA sequencing to
determine unknown PCR amplified sequence in which and of the amplification
primers may be used in sanger sequencing.
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PCR amplifies a specific region of a
DNA strand.
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Most PCR method amplify DNA fragments
of between 0.1 and 10 kilo base pairs in length.
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The basic PCR set-up requires several
component and reagent including a DNA template that contains the DNA target
region to amplify.
There are
many types of PCR technique
1.
Colony PCR
2.
Nested PCR
3.
Inverse PCR
4.
QT PCR
5.
Real time PCR
Normal concept of PCR
Question: -
At what temperature does the denature step of PCR
occur?
1. 1. 950c
2. 2. 720c
3. 3. 650c
4. 4. Any temperature
Answer write in Comment BOX
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